Isolation and identification of lactic acid bacteria producing β glucosidase from Indonesian fermented foods

Indonesia has many kind of fermented foods such as tempe, fermented rice, fermented cassava, and fermented vegetable. Lactic acid bacteria that can produce β glycosidase most likely in the fermented foods from plants materials. The objectives of the research were to isolate lactic acid bacteria producing β glucosidase enzyme from Indonesian fermented foods and identify the isolates to their species. Twenty one isolates were identified using 3 different methods, including phenotypic and genotypic identification from Bergey’s manual of Systematic Bacteriology, API 50 CHL Kits, and 16S rRNA sequence based methods. Finally, 20 isolates were close related as Pediococcus acidilactici, P. pentosaceus and P. lolii AB362985.1 and one isolate identified as Lactobacillus pentosus-plantarum group.